M phase, also called
as cell division, is the most crucial and fundamental affair
of the eukaryotic cell cycle (Alberts
B et al.
, 2002
). After the chromosomes have
been replicated during the S phase, the sister chromatids are
separated and distributed into two daughter cells equally and
faithfully. Also, each daughter cell receives the almost average
and necessary intracellular constituents and organelles from
the mother cell. Generally, cell division consists of six stages,
including prophase, prometaphase, metaphase, anaphase, telophase
and cytokinesis. And the first five stages constituent mitosis
(Alberts
B et al.
, 2002
). During mitosis, numerous
proteins organize protein super-complexes at the three distinct
regions of centrosome
(Badano
JL et al.
, 2005
;
Doxsey S et al.
, 2005a
;
Doxsey
S et al.
, 2005b
;
Andersen JS et al.
, 2003
)
(spindle
pole
in budding yeast (Jaspersen
SL et al.
, 2004
)), kinetochore
/centromere
(Chan
GK et al.
, 2005
;
Fukagawa T et al.
, 2004
;
McAinsh
AD et al.
, 2003
;
Yao X et al.
, 2000
;
Yao X et al.
, 1997
;
Bloom
K et al.
, 2005
) and cleavage
furrow
/midbody
(D'Avino
PP et al.
, 2005
;
Burgess DR et al.
, 2005
;
Glotzer M et al.
, 2004
;
Skop
AR et al.
,2004
) (related or homolog structures
in plants and budding yeast called as phragmoplast
(Otegui
MS et al.
, 2005
;
Guertin DA et al., 2002
) and
bud neck
(Guertin
DA et al.
, 2002
), respectively) spatially
and temporally, orchestrating the accomplishment of cell division
process.
Although many proteins have been
identified to be localized on centrosome, kinetochore and/or midbody,
an integrated resource on this area still remains not to be available.
In this work, we have collected all proteins identified to be localized
on kinetochore, centrosome, and/or midbody from two fungi (S. cerevisiae
and S. pombe
) and five animals, including C. elegans
,
D. melanogaster
, X. laevis
, M. musculus
and
H. sapiens
. From the related literature of PubMed, numerous proteins
have been manually curated to be localized on at least one of the sub-cellular
localizations of kinetochore, centrosome and midbody. And to promise the
quality of data, based on the rationale of "Seeing is believing"
(Bloom
K et al.
, 2005
), these proteins have been unambiguously
observed under fluorescent microscope as directly supportive evidences.
Then an integrated and searchable database
M
iCroKit
- M
idbody, C
entrosome
and K
inetochore has been
established. The MiCroKit database is the first integrative resource to
pin point most of identified components and related scientific information
of midbody, centrosome and kinetochore.
The version 1.0 of M
iCroKit
database was set up on Nov. 2nd, 2005, containing 1,065 unique proteins.
The
MiCroKit version 2.0
was released on Jun. 5th, 2006,
with 1,120 entries.
Currently, the MiCroKit
3.0
database was updated on July 9, 2009, containing
1,489
unique protein
entries. The online service of MiCroKit 3.0 was implemented in PHP + MySQL
+ JavaScript. And the local packages of MiCroKit 3.0 were developed in
JAVA 1.5 (J2SE). The database will be updated routinely as new microkit
proteins are reported. The MiCroKit 3.0 is freely available
at: http://microkit.biocuckoo.org
.
MiCroKit 3.0 User Interface
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